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96 well plate with 1 5 glass like polymer coverslip bottom  (Cellvis Inc)

 
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    Structured Review

    Cellvis Inc 96 well plate with 1 5 glass like polymer coverslip bottom
    96 Well Plate With 1 5 Glass Like Polymer Coverslip Bottom, supplied by Cellvis Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glass+bottom/coated+d+lysine+poly/pmc13199755-34-0-10
    Average 86 stars, based on 1 article reviews
    96 well plate with 1 5 glass like polymer coverslip bottom - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    High Content Screening:

    Article Title: Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12.
    Article Snippet: GFP+mCherry+ tumour cells were detected at 488 nm and 543 nm wavelengths respectively in sections using a laser scanning confocal microscopy (LSM800, Zeiss). .. To investigate the protein levels and localization of MDA5/ZBP1, EO771 cells were grown on a 24 well high content screening microplates with glass bottom (Cellvis, #P24-0-N) and treated with vehicle (2% DMSO in saline) or 100 nM 12-HETE (Cayman, #34570) for 48 h. After being washed, the cells were fixed, blocked in 5% normal goat serum for 1 h and stained with the primary antibody: rabbit anti-human/mouse MDA5 (1:100, Abcam, #ab79055), rabbit anti-human/mouse ZBP1 (1:100, Thermo Fisher Scientific, #PA5-20455) and mouse anti-J2 (1:100, Sigma Aldrich, #MABE1134) overnight at 4 °C. .. The Alexa Fluor secondary antibodies (1:300, Thermo Fisher Scientific, #A31570, #A-11034) were incubated for 1 h at room temperature.

    Article Title: Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12.
    Article Snippet: Briefly, the mCherry-Cry2 and IDR (ADAR1 p1501-250)-mCherry-Cry2 expression lentivirus were constructed by Genechem Company (Shanghai, China) and transduced into HEK293T cells at MOI of 10 as described above. .. Cells were transferred to a 96 well high content screening microplates with glass bottom (Cellvis, #P96-0-N) for another 24 h. Confocal images were acquired on a Zeiss LSM880. ..

    Article Title: Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12.
    Article Snippet: .. Colocalization of ADAR1 p150 and dsRNA in EO771 cells AR TI CL E IN P RE SS Endogenously GFP-tagged ADAR1 p150 EO771 cells were grown on a 24 well high content screening microplates with glass bottom (Cellvis, #P24-0-N) and treated with 100 nM 12-HETE (Cayman, #34570) or 12-HETE with 3% 1,6-hexanediol (Sigma Aldrich, #240117) for 48 h. After being washed, cells were fixed, permeabilized and stained with J2 the primary antibody (1:100, Sigma Aldrich, #MABE1134) overnight at 4 °C and then the anti-mouse Alexa-555 secondary antibody (1:300, Thermo Fisher Scientific, #A31570) for 1 h at room temperature. .. Confocal images were acquired on a Zeiss LSM880.

    Saline:

    Article Title: Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12.
    Article Snippet: GFP+mCherry+ tumour cells were detected at 488 nm and 543 nm wavelengths respectively in sections using a laser scanning confocal microscopy (LSM800, Zeiss). .. To investigate the protein levels and localization of MDA5/ZBP1, EO771 cells were grown on a 24 well high content screening microplates with glass bottom (Cellvis, #P24-0-N) and treated with vehicle (2% DMSO in saline) or 100 nM 12-HETE (Cayman, #34570) for 48 h. After being washed, the cells were fixed, blocked in 5% normal goat serum for 1 h and stained with the primary antibody: rabbit anti-human/mouse MDA5 (1:100, Abcam, #ab79055), rabbit anti-human/mouse ZBP1 (1:100, Thermo Fisher Scientific, #PA5-20455) and mouse anti-J2 (1:100, Sigma Aldrich, #MABE1134) overnight at 4 °C. .. The Alexa Fluor secondary antibodies (1:300, Thermo Fisher Scientific, #A31570, #A-11034) were incubated for 1 h at room temperature.

    Staining:

    Article Title: Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12.
    Article Snippet: GFP+mCherry+ tumour cells were detected at 488 nm and 543 nm wavelengths respectively in sections using a laser scanning confocal microscopy (LSM800, Zeiss). .. To investigate the protein levels and localization of MDA5/ZBP1, EO771 cells were grown on a 24 well high content screening microplates with glass bottom (Cellvis, #P24-0-N) and treated with vehicle (2% DMSO in saline) or 100 nM 12-HETE (Cayman, #34570) for 48 h. After being washed, the cells were fixed, blocked in 5% normal goat serum for 1 h and stained with the primary antibody: rabbit anti-human/mouse MDA5 (1:100, Abcam, #ab79055), rabbit anti-human/mouse ZBP1 (1:100, Thermo Fisher Scientific, #PA5-20455) and mouse anti-J2 (1:100, Sigma Aldrich, #MABE1134) overnight at 4 °C. .. The Alexa Fluor secondary antibodies (1:300, Thermo Fisher Scientific, #A31570, #A-11034) were incubated for 1 h at room temperature.

    Article Title: Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12.
    Article Snippet: .. Colocalization of ADAR1 p150 and dsRNA in EO771 cells AR TI CL E IN P RE SS Endogenously GFP-tagged ADAR1 p150 EO771 cells were grown on a 24 well high content screening microplates with glass bottom (Cellvis, #P24-0-N) and treated with 100 nM 12-HETE (Cayman, #34570) or 12-HETE with 3% 1,6-hexanediol (Sigma Aldrich, #240117) for 48 h. After being washed, cells were fixed, permeabilized and stained with J2 the primary antibody (1:100, Sigma Aldrich, #MABE1134) overnight at 4 °C and then the anti-mouse Alexa-555 secondary antibody (1:300, Thermo Fisher Scientific, #A31570) for 1 h at room temperature. .. Confocal images were acquired on a Zeiss LSM880.

    Transfection:

    Article Title: Integrative genomic and functional analyses reveal NINL as a modulator of tau aggregation
    Article Snippet: .. Twenty‐four hours after transfection, cells were replated at a density of 200,000 cells per well into 35 mm live imaging dishes with a 20 mm glass bottom well (Cellvis, Cat #: D35‐20‐1.5‐N) coated with Poly‐D‐Lysine. ..

    Imaging:

    Article Title: Integrative genomic and functional analyses reveal NINL as a modulator of tau aggregation
    Article Snippet: .. Twenty‐four hours after transfection, cells were replated at a density of 200,000 cells per well into 35 mm live imaging dishes with a 20 mm glass bottom well (Cellvis, Cat #: D35‐20‐1.5‐N) coated with Poly‐D‐Lysine. ..

    Article Title: Steric shielding of the KRAS4B hypervariable region enables isoform-specific inhibition of prenylation
    Article Snippet: .. The cultures were maintained at 37 °C with 5% CO2 in an imaging dish with a glass bottom (D35-14-1.5-N, Cellvis). .. One hour before injection, the medium was replaced with Live-Cell Imaging Solution (LCIS) (A14291DJ, Thermo Fisher Scientific) containing 10% FCS and penicillin/streptomycin.

    Multiple Displacement Amplification:

    Article Title: A shark VNAR-IL-15 fusion protein that suppresses TROP2 + tumor growth.
    Article Snippet: Interleukin-15 (IL-15) holds great promise for cancer immunotherapy because it selectively activates cytotoxic immune cells without expanding regulatory T cells.. It also has the potential to convert “cold” tumors into more immunologically active ones.. However, its clinical utility is limited by systemic toxicity.

    Cell Culture:

    Article Title: A shark VNAR-IL-15 fusion protein that suppresses TROP2 + tumor growth.
    Article Snippet: Interleukin-15 (IL-15) holds great promise for cancer immunotherapy because it selectively activates cytotoxic immune cells without expanding regulatory T cells.. It also has the potential to convert “cold” tumors into more immunologically active ones.. However, its clinical utility is limited by systemic toxicity.

    In Situ:

    Article Title: Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects.
    Article Snippet: After selection, cells were recovered for at least 2 days prior to freezing in media with 5% v/v added DMSO (Sigma, D8418-50 mL). .. All in situ sequencing experiments were performed using all wells of a glass-bottom #1.5H 6-well plate (Cellvis, P06-1.5H-N) with cells plated at 800,000 cells per well (U2OS) or 600,000 cells per well (iPS cell/NGN2-induced neuron), and unless indicated otherwise wash steps contain 2mL per well. .. ll OPEN ACCESS Cell 189, 1–19.e1–e13, June 25, 2026 e6 Please cite this article in press as: Pendyala et al., Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects, Cell (2026), https://doi.org/10.1016/j.cell.2026.04.031 Article For low-MOI integration of VIS-seq libraries into iPS cells, cells were electroporated in 2 cuvettes of Lonza 4D-Nucleofector P3 Kit X (Lonza, V4XP-3024) using the Lonza 4D Nucleofector with protocol CM104 (U2OS) or CM137 (iPS cells).

    Sequencing:

    Article Title: Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects.
    Article Snippet: After selection, cells were recovered for at least 2 days prior to freezing in media with 5% v/v added DMSO (Sigma, D8418-50 mL). .. All in situ sequencing experiments were performed using all wells of a glass-bottom #1.5H 6-well plate (Cellvis, P06-1.5H-N) with cells plated at 800,000 cells per well (U2OS) or 600,000 cells per well (iPS cell/NGN2-induced neuron), and unless indicated otherwise wash steps contain 2mL per well. .. ll OPEN ACCESS Cell 189, 1–19.e1–e13, June 25, 2026 e6 Please cite this article in press as: Pendyala et al., Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects, Cell (2026), https://doi.org/10.1016/j.cell.2026.04.031 Article For low-MOI integration of VIS-seq libraries into iPS cells, cells were electroporated in 2 cuvettes of Lonza 4D-Nucleofector P3 Kit X (Lonza, V4XP-3024) using the Lonza 4D Nucleofector with protocol CM104 (U2OS) or CM137 (iPS cells).



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